Search results for " antibiotic production"
showing 6 items of 6 documents
Elucidating the molecular physiology of lantibiotic NAI-107 production in Microbispora ATCC-PTA-5024.
2016
Background The filamentous actinomycete Microbispora ATCC-PTA-5024 produces the lantibiotic NAI-107, which is an antibiotic peptide effective against multidrug-resistant Gram-positive bacteria. In actinomycetes, antibiotic production is often associated with a physiological differentiation program controlled by a complex regulatory and metabolic network that may be elucidated by the integration of genomic, proteomic and bioinformatic tools. Accordingly, an extensive evaluation of the proteomic changes associated with NAI-107 production was performed on Microbispora ATCC-PTA-5024 by combining two-dimensional difference in gel electrophoresis, mass spectrometry and gene ontology approaches. R…
Antimicrobial and Insecticidal: Cyclic Lipopeptides and Hydrogen Cyanide Produced by Plant-Beneficial Pseudomonas Strains CHA0, CMR12a, and PCL1391 C…
2017
Particular groups of plant-beneficial fluorescent pseudomonads are not only root colonizers that provide plant disease suppression, but in addition are able to infect and kill insect larvae. The mechanisms by which the bacteria manage to infest this alternative host, to overcome its immune system, and to ultimately kill the insect are still largely unknown. However, the investigation of the few virulence factors discovered so far, points to a highly multifactorial nature of insecticidal activity. Antimicrobial compounds produced by fluorescent pseudomonads are effective weapons against a vast diversity of organisms such as fungi, oomycetes, nematodes, and protozoa. Here, we investigated whe…
THE SMALL PROTEIN TRPM MODULATES MORPHO-PHYSIOLOGICAL DIFFERENTIATION IN THE MODEL ACTINOMYCETE STREPTOMYCES COELICOLOR A3(2)
2017
BACKGROUNDS TrpM, a small protein of 63 amino acids, modulates tryptophan (Trp) metabolism and morpho-physiological differentiation in the filamentous bacterium Streptomyces coelicolor A3(2), a model organism for antibiotic production and cell differentiation. Indeed, the trpM knock-out mutant strain is characterized by a delayed growth on minimal medium, smaller aerial hyphae, and reduction of both spore and antibiotic actinorhodin production in comparison with the wild-type strain. These observations were in agreement with proteomic analyses which highlighted a role for TrpM in controlling i) Trp production through Trp precursor availability and, thus ii) bacterial growth and morpho-physi…
The small protein TrpM modulates morpho-physiological differentiation in Streptomyces coelicolor
2017
TrpM, a small protein of 63 amino acids, is encoded by a gene of the trpCMBA locus involved in tryptophan biosynthesis in the model actinomycete Streptomyces coelicolor. Indeed, the trpM knock-out mutant strain is characterized by a delayed growth on minimal medium, smaller aerial hyphae, and reduction of both spore and antibiotic actinorhodin production in comparison with the wild-type strain. These observations are in agreement with proteomic analyses which highlighted a role for TrpM in controlling i) tryptophan production through precursor availability and, thus ii) bacterial growth and morpho-physiological differentiation. To further elucidate the role of TrpM, a S. coelicolor trpM kno…
A small protein is involved in tryptophan biosynthesis and morpho-physiological differentiation in Streptomyces coelicolor
2015
A small protein is involved in tryptophan biosynthesis and morpho-physiological differentiation in Streptomyces coelicolor
From tryptophan metabolism to peptide antibiotic production in Streptomyces coelicolor
2009
The tryptophan is a precursor of the lipopeptide calcium dependent antibiotic (CDA), produced from Streptomyces coelicolor and closely related to important antibiotics such as daptomycin. We have focused our attention on the correlation between CDA production and tryptophan metabolism in order to identify new strategies aimed at increasing the production of peptide antibiotics.